Indutores de resistência no controle da cercosporiose do cafeeiro: análise de genes relacionados à defesa

SANDRA ELIZA GUIMARÃES; MÁRIO LÚCIO RESENDE; DEILA DOS SANTOS; ANA CRISTINA MONTEIRO; VICTOR AUGUSTO VASCONCELOS; MANOEL BATISTA JÚNIOR

doi:10.25186/cs.v11i3.1071

Authors

SANDRA ELIZA GUIMARÃES UNIVERSIDADE FEDERAL DE LAVRAS
MÁRIO LÚCIO RESENDE UNIVERSIDADE FEDERAL DE LAVRAS
DEILA DOS SANTOS UNIVERSIDADE FEDERAL DE LAVRAS
ANA CRISTINA MONTEIRO UNIVERSIDADE FEDERAL DE LAVRAS
VICTOR AUGUSTO VASCONCELOS UNIVERSIDADE FEDERAL DE LAVRAS
MANOEL BATISTA JÚNIOR UNIVERSIDADE FEDERAL DE LAVRAS

DOI:

https://doi.org/10.25186/cs.v11i3.1071

Keywords:

Resistance induction, oxygen reactive species, PR-protein, lipid oxidation

Abstract

The objective of this study was to evaluate the effect of resistance inducers, plant extract-based formulations and fungicide over the protection of coffee seedlings in controlling Cercospora coffeicola. In addition, the objective was to select alternative treatments that highlight in control of Cercospora leaf spot and study its effect as resistance inducer through the defense gene expression analysis. The treatments applied were: Greenforce CuCa, Greenforce KP, ET64-DT, which are plant extracts-based formulations (are under patent secrecy - PI 0603575-2), mineral fertilizer, acibenzolar-S-methyl (ASM) and, as control, the epoxiconazole + pyraclostrobin fungicide. We conducted weekly evaluations of incidence of cercosporiose to calculate the area under the curve of disease incidence progress (AUCDIP). After analysis of this experiment, the two best alternative treatments to evaluate the resistance-inducing potential of them were chosen. For this, a second experiment was carried out in order to analyze the expression of genes that are translated into the lipoxygenase, catalase, glutathione peroxidase and chitinase enzymes. The application of epoxiconazole + pyraclostrobin fungicide resulted in more effective control of the incidence of the disease, differing from the other treatments tested. The Greenforce Cuca and ASM, 0.05 g.L-1, products resulted in a reduction of AUCDIP of cercosporiose by 38 and 35%, respectively, differing from the other treatments. In the analysis of gene response expression to induce, it was observed that the highest expression of the genes encoding the lipoxygenase, catalase, glutathione peroxidase and chitinase enzymes occurred at 24 hours after application of ASM and 48 hours for Greenforce Cuca, no difference from one treatment to the other in these periods. Plants were sprayed with Greenforce Cuca showed a higher expression of the lipoxygenase gene at 72 hours after application of this product, differed from the ASM. With this work, we found that Greenforce Cuca and ASM products control Cercospora leaf spot through the induction of defense genes in coffee.

Author Biographies

SANDRA ELIZA GUIMARÃES, UNIVERSIDADE FEDERAL DE LAVRAS

BIÓLOGA, DOUTORANDA DO DEPARTAMENTO DE FITOPATOLOGIA, UNIVERSIDDADE FEDERAL DE LAVRAS, CAIXA POSTAL: 3037

MÁRIO LÚCIO RESENDE, UNIVERSIDADE FEDERAL DE LAVRAS

AGRÔNOMO, PROFESSOR DO DEPARTAMENTO DE FITOPATOLOGIA DA UNIVERSIDADE FEDERAL DE LAVRAS

DEILA DOS SANTOS, UNIVERSIDADE FEDERAL DE LAVRAS

AGRÔNOMA, PÓS-DOUTORANDA DO DEPARTAMENTO DE FITOPATOLOGIA DA UNIVERSIDADE FEDERAL DE LAVRAS

ANA CRISTINA MONTEIRO, UNIVERSIDADE FEDERAL DE LAVRAS

ENGENHEIRA AGRÔNOMA, PÓS-DOUTORANDA EM FITOPATOLOGIA UNIVERSIDADE FEDERAL DE LAVRAS

VICTOR AUGUSTO VASCONCELOS, UNIVERSIDADE FEDERAL DE LAVRAS

AGRONOMIA NA UNIVERSIDADE FEDERAL DE LAVRAS

MANOEL BATISTA JÚNIOR, UNIVERSIDADE FEDERAL DE LAVRAS

AGRÔNOMO, DOUTORANDO NO DEPARTAMENTO DE FITOPATOLOGIA DA UNIVERSIDADE FEDERAL DE LAVRAS

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Resistance inductors in control of coffee cercosporiose: analysis of genes related to defense